Potato dextrose broth
Potato dextrose broth (abbreviated as "PDB") and potato dextrose agar (abbreviated as "PDA") are common microbiological media for culturing both yeast and mold, but usually not bacteria. (2)
Common organisms that can be cultured on PDA are yeasts such as Candida albicans and Saccharomyces cerevisiae and molds such as Aspergillus niger. (3)
Preparation
PDB can be prepared by boiling a mixture of :
- peeled, finely chopped potatos (200g)
- dextrose (20g)
In 300ml of water for 30 minutes. The supernatant is then decanted and strained through cloth to remove particles from the broth. (1)
The final solution is a light yellow translucent broth. Because it is not sterile the broth may then need to be sterilized in an autoclave.
References
1. Atlas, R.M.: Handbook of Microbiological Media, second edition. Lawrence C. Parks (1997)
2. Harold Eddleman, Ph. D: Bacteria Media From Potato. Jan 1998. [1]. Accessed 29 May 2005.
3. Merck KGaA: Potato Dextrose Broth. 2002. [2]. Accessed 29 May 2005.